Development and cytotoxic response of two proliferative MDA- MB-231 and non-proliferative SUM1315 three-dimensional cell culture models of triple-negative basal-like breast cancer cell lines

摘要

Triple-Negative Basal-Like tumors, representing 15 to 20% of breast cancers,are very aggressive and with poor prognosis. Targeted therapies have beendeveloped extensively in preclinical and clinical studies to open the way fornew treatment strategies. The present study has focused on developing 3D cellcultures from SUM1315 and MDA-MB-231, two triple-negative basal-like (TNBL)breast cancer cell lines, using the liquid overlay technique. Extracellularmatrix concentration, cell density, proliferation, cell viability, topology andultrastructure parameters were determined. The results showed that for bothcell lines, the best conditioning regimen for compact and homogeneous spheroidformation was to use 1000 cells per well and 2% Geltrex. This conditioningregimen highlighted two 3D cell models: non-proliferative SUM1315 spheroids andproliferative MDA-MB-231 spheroids. In both cell lines, the comparison of 2D vs3D cell culture viability in the presence of increasing concentrations ofchemotherapeutic agents i.e. cisplatin, docetaxel and epirubicin, showed thatspheroids were clearly less sensitive than monolayer cell cultures. Moreover, aproliferative or non-proliferative 3D cell line property would enabledetermination of cytotoxic and/or cytostatic drug activity. 3D cell culturecould be an excellent tool in addition to the arsenal of techniques currentlyused in preclinical studies. http://www.impactjournals.com/oncotarget/Oncotarget, Advance Publications 2017